Rabbit MACA Polyclonal Antibody | anti-MACA antibody

Rabbit anti-Escherichia coli (strain K12) MACA Polyclonal Antibody

• Gene Names: macA; ECK0869; JW0862; ybjY
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: MACA; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) MACA Polyclonal Antibody; MACA Antibody; Macrolide export protein MacA macA ybjY b0878 JW0862; anti-MACA antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-MACA antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) macA protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 90111182
• NCBI GeneID: 947322
• NCBI Accession #: NP_415399.4
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P75830
• Molecular Weight: 40,624 Da
• NCBI Official Full Name: macrolide transporter membrane fusion protein (MFP) component
• NCBI Official Symbol: macA
• NCBI Official Synonym Symbols: ECK0869; JW0862; ybjY
• NCBI Protein Information: macrolide transporter membrane fusion protein (MFP) component
• UniProt Protein Name: Macrolide export protein MacA
• Protein Family: Maleylacetate reductase
• UniProt Gene Name: macA
• UniProt Synonym Gene Names: ybjY

NCBI Description

OmpF consistently co-purifies with MacA; MacA stimulates MacB ATPase activity (Tikhonova, 2007). [More information is available at EcoGene: EG13694]. MacA is a peripheral membrane protein of the MFP family and a component of the MacAB macrolide efflux transporter complex. [More information is available at EcoCyc: G6461].

Uniprot Description

Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.