Rabbit VPU Polyclonal Antibody | anti-VPU antibody

Rabbit anti-Human immunodeficiency virus type 1 group M subtype B (isolate HXB3)(HIV-1) VPU Polyclonal Antibody

• Reactivity: Human immunodeficiency virus type 1 group M subtype B (isolate HXB3)(HIV-1)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: VPU; Polyclonal Antibody; Rabbit anti-Human immunodeficiency virus type 1 group M subtype B (isolate HXB3)(HIV-1) VPU Polyclonal Antibody; VPU Antibody; Protein Vpu; U ORF protein; Viral protein U; vpu; anti-VPU antibody

• Host: Rabbit
• Reactivity: Human immunodeficiency virus type 1 group M subtype B (isolate HXB3)(HIV-1)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-VPU antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Human immunodeficiency virus type 1 group M subtype B (isolate HXB3) (HIV-1) vpu protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 62291037
• UniProt Accession #: P69700; P05920
• Molecular Weight: 9,174 Da
• NCBI Official Full Name: Protein Vpu
• UniProt Protein Name: Protein Vpu
• Protein Family: Protein
• UniProt Gene Name: vpu

Uniprot Description

Enhances virion budding by targeting host CD4 and Tetherin/BST2 to proteasome degradation. Degradation of CD4 prevents any unwanted premature interactions between viral Env and its host receptor CD4 in the endoplasmic reticulum. Degradation of antiretroviral protein Tetherin/BST2 is important for virion budding, as BST2 tethers new viral particles to the host cell membrane. Mechanistically, Vpu bridges either CD4 or BST2 to BTRC, a substrate recognition subunit of the Skp1/Cullin/F-box protein E3 ubiquitin ligase, induces their ubiquitination and subsequent proteasomal degradation. The alteration of the E3 ligase specificity by Vpu seems to promote the degradation of host IKBKB, leading to NF-kappa-B down-regulation and subsequent apoptosis. Ion channel activity has also been suggested, however, formation of cation-selective channel has been reconstituted ex-vivo in lipid bilayers. It is thus unsure that this activity plays a role in vivo.