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SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-4: K562 cells, 231 cells, Hela cells, Human fetal brain tissue, Primary antibody: MBS7126834(RHOA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes)

Rabbit RHOA Polyclonal Antibody | anti-RHOA antibody

RHOA Antibody

Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Antigen Affinity Purification
Synonyms
RHOA; Polyclonal Antibody; RHOA Antibody; Ras homolog family member A; anti-RHOA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol
Applicable Applications for anti-RHOA antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
Immunogen
Synthetic peptide of human RHOA
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

SDS-PAGE

(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-4: K562 cells, 231 cells, Hela cells, Human fetal brain tissue, Primary antibody: MBS7126834(RHOA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes)

SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-4: K562 cells, 231 cells, Hela cells, Human fetal brain tissue, Primary antibody: MBS7126834(RHOA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes)
Product Categories/Family for anti-RHOA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
387
UniProt Accession #
Molecular Weight
193
NCBI Official Full Name
Transforming protein RhoA
NCBI Official Synonym Full Names
ras homolog family member A
NCBI Official Symbol
RHOA
NCBI Official Synonym Symbols
ARHA; ARH12; RHO12; RHOH12
NCBI Protein Information
transforming protein RhoA; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A
UniProt Protein Name
Transforming protein RhoA
Protein Family
UniProt Gene Name
RHOA
UniProt Synonym Gene Names
ARH12; ARHA; RHO12; h12
UniProt Entry Name
RHOA_HUMAN

Uniprot Description

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cytosol; cell junction; cleavage furrow

Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; transforming growth factor beta receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation

Research Articles on RHOA

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Product Notes

The RHOA rhoa (Catalog #AAA7126834) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHOA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHOA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:1000-1:5000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RHOA rhoa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHOA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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