Immunohistochemistry (IHC)
(IHC image of CSB-PA839291LA01HU diluted at 1:600 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit anti-Human MIB2 Polyclonal Antibody | anti-MIB2 antibody
MIB2 Antibody
Gene Names
MIB2; ZZZ5; ZZANK1
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
>95%Protein G Purified
Synonyms
MIB2; Polyclonal Antibody; MIB2 Antibody; E3 ubiquitin-protein ligase MIB2; EC 2.3.2.27; Mind bomb homolog 2; Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; RING-type E3 ubiquitin transferase MIB2; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1; SKD ZZANK1; anti-MIB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Protein G Purified
Form/Format
Liquid
Sequence Length
1066
Applicable Applications for anti-MIB2 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC: 1:500-1:1000
Immunogen Species
Human
Immunogen
Recombinant Human E3 ubiquitin-protein ligase MIB2 protein (1-128AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
Immunohistochemistry (IHC)
(IHC image of CSB-PA839291LA01HU diluted at 1:600 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of CSB-PA839291LA01HU diluted at 1:600 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Product Categories/Family for anti-MIB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
108,404 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MIB2 isoform 2
NCBI Official Synonym Full Names
mindbomb E3 ubiquitin protein ligase 2
NCBI Official Symbol
MIB2
NCBI Official Synonym Symbols
ZZZ5; ZZANK1
NCBI Protein Information
E3 ubiquitin-protein ligase MIB2
UniProt Protein Name
E3 ubiquitin-protein ligase MIB2
Protein Family
UniProt Gene Name
MIB2
UniProt Synonym Gene Names
SKD; ZZANK1; Novelzin
NCBI Description
The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017]
Uniprot Description
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.
Research Articles on MIB2
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Product Notes
The MIB2 mib2 (Catalog #AAA7114294) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIB2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIB2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). IHC: 1:500-1:1000. Researchers should empirically determine the suitability of the MIB2 mib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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