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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, A549 whole cell lysateAll lanes: DNAJA3 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 50, 34 kDaObserved band size: 53 kDa)

Rabbit anti-Human DNAJA3 Polyclonal Antibody | anti-DNAJA3 antibody

DNAJA3 Antibody

Gene Names
DNAJA3; TID1; HCA57; hTID-1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%
Protein G Purified
Synonyms
DNAJA3; Polyclonal Antibody; DNAJA3 Antibody; DnaJ homolog subfamily A member 3; mitochondrial; DnaJ protein Tid-1; hTid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog; HCA57 TID1; anti-DNAJA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
453
Applicable Applications for anti-DNAJA3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IHC: 1:200-1:500
IF: 1:50-1:200
Immunogen Species
Human
Immunogen
Recombinant Human DnaJ homolog subfamily A member 3, mitochondrial protein (86-184AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, A549 whole cell lysateAll lanes: DNAJA3 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 50, 34 kDaObserved band size: 53 kDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, A549 whole cell lysateAll lanes: DNAJA3 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 50, 34 kDaObserved band size: 53 kDa)

Immunohistochemistry (IHC)

(IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of MCF-7 cells with CSB-PA836207LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of MCF-7 cells with CSB-PA836207LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-DNAJA3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,611 Da
NCBI Official Full Name
dnaJ homolog subfamily A member 3, mitochondrial isoform 2
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member A3
NCBI Official Symbol
DNAJA3
NCBI Official Synonym Symbols
TID1; HCA57; hTID-1
NCBI Protein Information
dnaJ homolog subfamily A member 3, mitochondrial
UniProt Protein Name
DnaJ homolog subfamily A member 3, mitochondrial
Protein Family
UniProt Gene Name
DNAJA3
UniProt Synonym Gene Names
HCA57; TID1; hTid-1

NCBI Description

This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.

Research Articles on DNAJA3

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Product Notes

The DNAJA3 dnaja3 (Catalog #AAA7114266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJA3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJA3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:200-1:500 IF: 1:50-1:200. Researchers should empirically determine the suitability of the DNAJA3 dnaja3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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