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Immunofluorescence (IF) (Immunofluorescence staining of SH-SY5Y cells with CSB-PA415667LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Rabbit anti-Human ARHGAP32 Polyclonal Antibody | anti-ARHGAP32 antibody

ARHGAP32 Antibody

Gene Names
ARHGAP32; GRIT; RICS; GC-GAP; PX-RICS; p250GAP; p200RhoGAP
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%
Protein G Purified
Synonyms
ARHGAP32; Polyclonal Antibody; ARHGAP32 Antibody; Rho GTPase-activating protein 32; Brain-specific Rho GTPase-activating protein; GAB-associated Cdc42/Rac GTPase-activating protein; GC-GAP; GTPase regulator interacting with TrkA; Rho-type GTPase-activating protein 32; Rho/Cdc42/Rac GTPase-activating protein RICS; RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling; p200RhoGAP; p250GAP; GRIT; KIAA0712; RICS; anti-ARHGAP32 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
2087
Applicable Applications for anti-ARHGAP32 antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:20-1:200
Immunogen Species
Human
Immunogen
Recombinant Human Rho GTPase-activating protein 32 protein (130-205AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescence staining of SH-SY5Y cells with CSB-PA415667LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of SH-SY5Y cells with CSB-PA415667LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-ARHGAP32 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
190,970 Da
NCBI Official Full Name
rho GTPase-activating protein 32 isoform 1
NCBI Official Synonym Full Names
Rho GTPase activating protein 32
NCBI Official Symbol
ARHGAP32
NCBI Official Synonym Symbols
GRIT; RICS; GC-GAP; PX-RICS; p250GAP; p200RhoGAP
NCBI Protein Information
rho GTPase-activating protein 32
UniProt Protein Name
Rho GTPase-activating protein 32
UniProt Gene Name
ARHGAP32
UniProt Synonym Gene Names
GRIT; KIAA0712; RICS

NCBI Description

RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008]

Uniprot Description

GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity ().

Research Articles on ARHGAP32

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Product Notes

The ARHGAP32 arhgap32 (Catalog #AAA7113830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP32 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP32 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:20-1:200. Researchers should empirically determine the suitability of the ARHGAP32 arhgap32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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