Rabbit anti-Human RASSF3 Polyclonal Antibody | anti-RASSF3 antibody

RASSF3 Antibody

Cat. Num. AAA7109312

• Gene Names: RASSF3; RASSF5
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: RASSF3; Polyclonal Antibody; RASSF3 Antibody; Ras association domain-containing protein 3; anti-RASSF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 238

• Applicable Applications for anti-RASSF3 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:200-1:500; IF: 1:50-1:200
• Immunogen: Recombinant Human Ras association domain-containing protein 3 protein (2-109AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(IHC image of MBS7109312 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of A549 cells with MBS7109312 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-RASSF3 antibody

cytosol, plasma membrane, identical protein binding

Product Categories/Family for anti-RASSF3 antibody

Cancer; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 30039698
• NCBI GeneID: 283349
• NCBI Accession #: NP_835463.1
• NCBI GenBank Nucleotide #: NM_178169.3
• UniProt Accession #: Q86WH2; Q86WH1
• Molecular Weight: 10,265 Da
• NCBI Official Full Name: ras association domain-containing protein 3
• NCBI Official Synonym Full Names: Ras association domain family member 3
• NCBI Official Symbol: RASSF3
• NCBI Official Synonym Symbols: RASSF5
• NCBI Protein Information: ras association domain-containing protein 3
• UniProt Protein Name: Ras association domain-containing protein 3
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF3

NCBI Description

The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]