Rabbit anti-Human RFXANK Polyclonal Antibody | anti-RFXANK antibody

RFXANK Antibody

Cat. Num. AAA7108055

• Gene Names: RFXANK; BLS; RFX-B; ANKRA1; F14150_1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: RFXANK; Polyclonal Antibody; RFXANK Antibody; DNA-binding protein RFXANK; Ankyrin repeat family A protein 1; Regulatory factor X subunit B; RFX-B; Regulatory factor X-associated ankyrin-containing protein; ANKRA1; RFXB; anti-RFXANK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 238

• Applicable Applications for anti-RFXANK antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:200-1:500; IF: 1:50-1:200
• Immunogen: Recombinant Human DNA-binding protein RFXANK protein (3-84AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysateAll lanes: RFXANK antibody at 6.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29, 25, 26 KDaObserved band size: 29 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7108055 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescence staining of PC3 cells with MBS7108055 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-RFXANK antibody

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed:9806546, PubMed:10072068, PubMed:10725724). May also potentiate the activation of RAF1 (By similarity).

Product Categories/Family for anti-RFXANK antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 523498361
• NCBI GeneID: 8625
• NCBI Accession #: NP_001265656.1
• NCBI GenBank Nucleotide #: NM_001278727.1
• UniProt Accession #: O14593; O95839; Q24JQ1; Q6FGA8
• Molecular Weight: 25,652 Da
• NCBI Official Full Name: DNA-binding protein RFXANK isoform c
• NCBI Official Synonym Full Names: regulatory factor X associated ankyrin containing protein
• NCBI Official Symbol: RFXANK
• NCBI Official Synonym Symbols: BLS; RFX-B; ANKRA1; F14150_1
• NCBI Protein Information: DNA-binding protein RFXANK
• UniProt Protein Name: DNA-binding protein RFXANK
• Protein Family: DNA-binding protein
• UniProt Gene Name: RFXANK
• UniProt Synonym Gene Names: ANKRA1; RFXB; RFX-B

NCBI Description

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed:9806546, PubMed:10072068, PubMed:10725724). May also potentiate the activation of RAF1 ().