Rabbit JUN Polyclonal Antibody | anti-JUN antibody

JUN Antibody

Cat. Num. AAA7103885

• Gene Names: JUN; AP1; p39; AP-1; c-Jun
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: JUN; Polyclonal Antibody; JUN Antibody; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; anti-JUN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 331

• Applicable Applications for anti-JUN antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IF: 1:50-1:200
• Immunogen: Recombinant Human Transcription factor AP-1 protein (1-100AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: NIH/3T3 whole cell lysate, HL-60 whole cell lysate, Rat brain tissue, Rat lung tissue, Rat stomach tissue, Mouse brain tissue, Mouse kidney tissueAll lanes: JUN antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS7103885 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-JUN antibody

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Product Categories/Family for anti-JUN antibody

Epigenetics and Nuclear Signaling; Cancer; Immunology; Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4758616
• NCBI GeneID: 3725
• NCBI Accession #: NP_002219.1
• NCBI GenBank Nucleotide #: NM_002228.3
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: transcription factor AP-1
• NCBI Official Synonym Full Names: Jun proto-oncogene, AP-1 transcription factor subunit
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; p39; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: Junctophilin
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).