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Typical Testing Data/Standard Curve (for reference only)

Rat chromosome 10 open reading frame 59 ELISA Kit | C10orf59 elisa kit

Rat Renalase/ Monoamine Oxidase-C, RNLS/ MAO-C ELISA Kit

Gene Names
RNLS; C10orf59; RENALASE
Reactivity
Rat
Synonyms
chromosome 10 open reading frame 59; Rat Renalase/ Monoamine Oxidase-C; RNLS/ MAO-C ELISA Kit; Rat Renalase/ Monoamine Oxidase-C (RNLS/ MAO-C) ELISA Kit; C10orf59; FLJ11218; RENALASE; renalase; C10orf59 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat RNLS. No significant cross-reactivity or interference between rat RNLS and analogues was observed.
Sequence Length
342
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
62.5 ng/ml -4000 ng/ml.
Sensitivity
The minimum detectable dose of rat RNLS is typically less than 15.6 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for C10orf59 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RNLS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNLS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNLS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNLS bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,847 Da
NCBI Official Full Name
renalase isoform 1
NCBI Official Synonym Full Names
renalase, FAD-dependent amine oxidase
NCBI Official Symbol
RNLS
NCBI Official Synonym Symbols
C10orf59; RENALASE
NCBI Protein Information
renalase; MAO-C; monoamine oxidase-C; alpha-NAD(P)H oxidase/anomerase
UniProt Protein Name
Renalase
UniProt Gene Name
RNLS
UniProt Synonym Gene Names
C10orf59; MAO-C
UniProt Entry Name
RNLS_HUMAN

NCBI Description

Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005 [PubMed 15841207]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Catalyzes the oxidation of the less abundant alpha-NAD(P)H isoform to form beta-NAD(P)+. The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.2 PublicationsManual assertion based on experiment in:Ref.4

Catalytic activity: Alpha-NAD(P)H + O2 = beta-NAD(P)+ + H2O2.1 PublicationManual assertion based on experiment in:Ref.6

Cofactor: FAD.1 PublicationManual assertion based on experiment in:Ref.7

Research Articles on C10orf59

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Product Notes

The Rat C10orf59 rnls (Catalog #AAA707117) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA707117 ELISA Kit recognizes Rat C10orf59. It is sometimes possible for the material contained within the vial of "chromosome 10 open reading frame 59, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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