Mouse suppressors of cytokine signaling 1 (SOCS-1) ELISA Kit | SOCS-1 elisa kit

Mouse suppressors of cytokine signaling 1, SOCS-1 ELISA Kit

Cat. Num. AAA705857

• Gene Names: Socs1; JAB; Cish1; Cish7; SSI-1; SOCS-1
• Reactivity: Mouse
• Synonyms: suppressors of cytokine signaling 1 (SOCS-1); Mouse suppressors of cytokine signaling 1; SOCS-1 ELISA Kit; Mouse suppressors of cytokine signaling 1 (SOCS-1) ELISA Kit; CIS1; CISH1; JAB; SOCS-1; SSI-1; SSI1; TIP3; JAK binding protein; STAT induced SH3 protein 1; Tec-interacting protein 3; cytokine-inducible SH2 protein 1; SOCS-1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SOCS-1. No significant cross-reactivity or interference between mouse SOCS-1 and analogues was observed.
• Sequence Length: 212

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml -4000 pg/ml
• Sensitivity: 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOCS-1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOCS-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOCS-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOCS-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOCS-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 409971432
• NCBI GeneID: 12703
• NCBI Accession #: NP_001258532.1
• NCBI GenBank Nucleotide #: NM_001271603.1
• UniProt Accession #: O35716; O35960; Q3U3L0; A2RT46
• Molecular Weight: 23,715 Da
• NCBI Official Full Name: suppressor of cytokine signaling 1
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 1
• NCBI Official Symbol: Socs1
• NCBI Official Synonym Symbols: JAB; Cish1; Cish7; SSI-1; SOCS-1
• NCBI Protein Information: suppressor of cytokine signaling 1; JAK2-binding protein; STAT-induced STAT inhibitor 1; cytokine inducible SH2-containing protein 1; cytokine inducible SH2-containing protein 7
• UniProt Protein Name: Suppressor of cytokine signaling 1
• UniProt Gene Name: Socs1
• UniProt Synonym Gene Names: Cish1; Ssi1; SOCS-1; JAB; SSI-1
• UniProt Entry Name: SOCS1_MOUSE

Uniprot Description

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2 SOCS1 appears to be a negative regulator in IGF1R signaling pathway 2 PublicationsManual assertion based on experiment in:Ref.14