Human CD68 molecule ELISA Kit | CD68 elisa kit

Human Cluster of Differentiation 68, CD68 ELISA Kit

Cat. Num. AAA705651

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Reactivity: Human
• Synonyms: CD68 molecule; Human Cluster of Differentiation 68; CD68 ELISA Kit; Human Cluster of Differentiation 68 (CD68) ELISA kit; DKFZp686M18236; GP110; SCARD1; CD68 antigen; macrophage antigen CD68; macrosialin; scavenger receptor class D; member 1; CD68 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CD68. No significant cross-reactivity or interference between Human CD68 and analogues was observed.
• Sequence Length: 327

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 15.6 pg/ml -1000 pg/ml
• Sensitivity: 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD68 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD68. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CD68 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CD68, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CD68 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 91199550
• NCBI GeneID: 968
• NCBI Accession #: NP_001035148.1
• NCBI GenBank Nucleotide #: NM_001040059.1
• UniProt Accession #: P34810; Q53HR6; Q53XI3; Q96BI7; B4DVT4
• Molecular Weight: 37,408 Da
• NCBI Official Full Name: macrosialin isoform B
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68
• UniProt Entry Name: CD68_HUMAN

NCBI Description

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.