Rabbit ABCC11 Polyclonal Antibody | anti-ABCC11 antibody

ABCC11 Polyclonal Antibody

Cat. Num. AAA3006389

• Gene Names: ABCC11; WW; EWWD; MRP8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ABCC11; Polyclonal Antibody; ABCC11 Polyclonal Antibody; ATP-binding cassette sub-family C member 11; Multidrug resistance-associated protein 8; MRP8; anti-ABCC11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ABCC11 polyclonal antibody detects endogenous levels of ABCC11 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1382

• Applicable Applications for anti-ABCC11 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ABCC11.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ABCC11 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCC11 pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCC11 pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCC11 pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCC11 pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ABCC11 antibody

ATP-binding cassette (ABC) transporters belong to an evolutionarily conserved family of proteins that catalyze the transport of molecules across extra- and intracellular membranes through the energy of ATP hydrolysis. ABC genes comprise seven subfamilies, designated ABC1, MDR/TAP, MRP, ALD, OABP, GCN20 and White. The complete human ABCC subfamily has 12 identified members (ABCC1-12), nine from the multidrug resistance-like subgroup, two from the sulfonylurea receptor subgroup, and the CFTR gene. The human ABCC11 gene maps to chromosome 16q12.1 and encodes a 1, 382 amino acid protein. The human ABCC12 gene maps to chromosome 16q12.1 and encodes a 1, 359 amino acid protein. Transcripts of ABCC11 and ABCC12 genes are present in various adult human tissues, including liver, lung and kidney, and also in several fetal tissues. Their chromosomal localization, potential function and expression patterns identify them as candidates for paroxysmal kinesigenic choreoathetosis, a disorder characterized by attacks of involuntary movements and postures, chorea and dystonia. Other inherited disorders where ABC transporters are implicated include cystic fibrosis, neurological disease, retinal degeneration, cholesterol and bile transport defects, anemia and drug response.

NCBI and Uniprot Product Information

• NCBI GI #: 21729873
• NCBI GeneID: 85320
• NCBI Accession #: NP_115972.2
• NCBI GenBank Nucleotide #: NP_115972.2
• UniProt Accession #: Q96J66
• Molecular Weight: ~ 125kDa
• NCBI Official Full Name: ATP-binding cassette sub-family C member 11 isoform a
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 11
• NCBI Official Symbol: ABCC11
• NCBI Official Synonym Symbols: WW; EWWD; MRP8
• NCBI Protein Information: ATP-binding cassette sub-family C member 11
• UniProt Protein Name: ATP-binding cassette sub-family C member 11
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC11
• UniProt Synonym Gene Names: MRP8

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. The product of this gene participates in physiological processes involving bile acids, conjugated steroids, and cyclic nucleotides. In addition, a SNP in this gene is responsible for determination of human earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides. Enhances the cellular extrusion of cAMP and cGMP. Stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E217betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. Probably functions to secrete earwax.