Mouse eosinophil peroxidase ELISA Kit | EPX elisa kit

Mouse Eosinophil Peroxidase, EPO ELISA Kit

Cat. Num. AAA705531

• Gene Names: Epx; EPO
• Reactivity: Mouse
• Synonyms: eosinophil peroxidase; Mouse Eosinophil Peroxidase; EPO ELISA Kit; Mouse Eosinophil Peroxidase (EPO) ELISA Kit; EPO; EPP; EPX-PEN; ; EPX elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse EPO. No significant cross-reactivity or interference between mouse EPO and analogues was observed.
• Sequence Length: 716

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 0.156 ng/ml-10 ng/ml.
• Sensitivity: 0.039 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPX elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 145966840
• NCBI GeneID: 13861
• NCBI Accession #: NP_031972.2
• NCBI GenBank Nucleotide #: NM_007946.2
• UniProt Accession #: P49290; Q5SW51; Q61798
• Molecular Weight: 81,380 Da
• NCBI Official Full Name: eosinophil peroxidase
• NCBI Official Synonym Full Names: eosinophil peroxidase
• NCBI Official Symbol: Epx
• NCBI Official Synonym Symbols: EPO
• NCBI Protein Information: eosinophil peroxidase
• UniProt Protein Name: Eosinophil peroxidase
• Protein Family: Eosinophil peroxidase
• UniProt Gene Name: Epx
• UniProt Synonym Gene Names: Eper; EPO
• UniProt Entry Name: PERE_MOUSE

Uniprot Description

Function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils 1 PublicationManual assertion based on experiment in:Ref.4

Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer