Human annexin A3 ELISA Kit | ANXA3 elisa kit

Human Annexin III, ANX-III ELISA Kit

Cat. Num. AAA704843

• Gene Names: ANXA3; ANX3
• Reactivity: Human
• Synonyms: annexin A3; Human Annexin III; ANX-III ELISA Kit; Human Annexin III (ANX-III) ELISA Kit; ANX3; Annexin III (lipocortin III) ; annexin III (lipocortin III; 1; 2-cyclic-inositol-phosphate phosphodiesterase; placental anticoagulant protein III; calcimedin 35-alpha) ; calcimedin 35-alpha; ANXA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ANXA3. No significant cross-reactivity or interference between Human ANXA3 and analogues was observed.
• Sequence Length: 323

• Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
• Detection Range: 0.78 ng/ml-50 ng/ml
• Sensitivity: 0.195 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANX-III. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANX-III. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANX-III., biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANX-III. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4826643
• NCBI GeneID: 306
• NCBI Accession #: NP_005130.1
• NCBI GenBank Nucleotide #: NM_005139.2
• UniProt Accession #: P12429; Q6LET2; B2R9W6
• Molecular Weight: 36,375 Da
• NCBI Official Full Name: annexin A3
• NCBI Official Synonym Full Names: annexin A3
• NCBI Official Symbol: ANXA3
• NCBI Official Synonym Symbols: ANX3
• NCBI Protein Information: annexin A3; PAP-III; annexin-3; lipocortin III; 35-alpha calcimedin; calcimedin 35-alpha; Annexin III (lipocortin III); placental anticoagulant protein III; inositol 1,2-cyclic phosphate 2-phosphohydrolase; annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)
• UniProt Protein Name: Annexin A3
• Protein Family: Annexin
• UniProt Gene Name: ANXA3
• UniProt Synonym Gene Names: ANX3; PAP-III
• UniProt Entry Name: ANXA3_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.