Rabbit anti-Mouse Ctcfl Polyclonal Antibody | anti-Ctcfl antibody

Ctcfl Antibody

Cat. Num. AAA7047961

• Gene Names: Ctcfl; Boris; OTTMUSG00000016680
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Ctcfl; Polyclonal Antibody; Ctcfl Antibody; Transcriptional repressor CTCFL; Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein; Boris; anti-Ctcfl antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 636

• Applicable Applications for anti-Ctcfl antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1: 500-2000
• Immunogen: Recombinant mouse Transcriptional repressor CTCFL protein (1-636AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Ctcfl antibody at 6ug/ml+Hela whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 74,75 kDaObserved band size: 73 kDa)

Related Product Information for anti-Ctcfl antibody

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

NCBI and Uniprot Product Information

• NCBI GI #: 124487069
• NCBI GeneID: 664799
• NCBI Accession #: NP_001074856.1
• NCBI GenBank Nucleotide #: NM_001081387.2
• UniProt Accession #: A2APF3; Q3Y6S0; B9EKP6
• Molecular Weight: 74,098 Da
• NCBI Official Full Name: transcriptional repressor CTCFL
• NCBI Official Synonym Full Names: CCCTC-binding factor (zinc finger protein)-like
• NCBI Official Symbol: Ctcfl
• NCBI Official Synonym Symbols: Boris; OTTMUSG00000016680
• NCBI Protein Information: transcriptional repressor CTCFL
• UniProt Protein Name: Transcriptional repressor CTCFL
• Protein Family: Transcriptional repressor
• UniProt Gene Name: Ctcfl
• UniProt Synonym Gene Names: Boris
• UniProt Entry Name: CTCFL_MOUSE

Uniprot Description

BORIS: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3. Testis specific. Specifically expressed in primary spermatocytes. Belongs to the CTCF zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Cancer Testis Antigen (CTA); DNA-binding

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; histone binding; protein binding; sequence-specific DNA binding

Biological Process: DNA methylation during gametogenesis; genetic imprinting; histone methylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of histone H3-K4 methylation