Typical Testing Data/Standard Curve (for reference only)
Human enolase 2 (gamma, neuronal) ELISA Kit | ENO2 elisa kit
Human Neuron-specific enolase, NSE ELISA Kit
Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human
Synonyms
enolase 2 (gamma; neuronal); Human Neuron-specific enolase; NSE ELISA Kit; NSE; 2-phospho-D-glycerate hydrolyase; enolase 2; neural enolase; neuron specific gamma enolase; neurone-specific enolase; ENO2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ENO2. No significant cross-reactivity or interference between Human ENO2 and analogues was observed.
Sequence Length
434
Samples
Serum, plasma, tissue homogenates
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.2 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
glyconeogenesis and glycometabolism
Protein Biological Process 3
Glycolysis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ENO2 elisa kit
Introduction: Neuron Specific Enolase (NSE) is the most abundant form of the glycolytic enolase found in adult neurons and is thought to serve as a growth factor in neurons. Of the three enolase subunits (alpha, beta, and gamma), NSE is a dimer composed of two gamma subunits. NSE is useful in studying neuronal differentiation and is, therefore, a valuable tool for visualizing the entire neuron and endocrine systems. Serum levels of NSE have been associated with such disease states as Alzheimer's, Huntington's Chorea, neuroblastoma, head trauma, neuroendocrine malignancies, and small cell carcinomas of the lung.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for NSE to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NSE, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NSE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for NSE to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NSE, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NSE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,269 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; epididymis secretory protein Li 279
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival UniRule annotationHAMAP-Rule:MF_00318
Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotationHAMAP-Rule:MF_00318
Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.
Research Articles on ENO2
Similar Products
Product Notes
The Human ENO2 eno2 (Catalog #AAA704796) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704796 ELISA Kit recognizes Human ENO2. It is sometimes possible for the material contained within the vial of "enolase 2 (gamma, neuronal), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
