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Western Blot (WB) (Western BlotPositive WB detected in:Hela whole cell lysate,HEK293 whole cell lysate,Mouse heart tissueAll lanes: ABLIM1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88,82,49,47,53,85 kDaObserved band size: 88 kDa)

Rabbit anti-Human, Mouse ABLIM1 Polyclonal Antibody | anti-ABLIM1 antibody

ABLIM1 Antibody

Gene Names
ABLIM1; ABLIM; LIMAB1; LIMATIN; abLIM-1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
ABLIM1; Polyclonal Antibody; ABLIM1 Antibody; Actin-binding LIM protein 1abLIM-1; Actin-binding LIM protein family member 1; Actin-binding double zinc finger protein; LIMAB1; Limatin; ABLIM; KIAA0059; anti-ABLIM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
718
Applicable Applications for anti-ABLIM1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant human Actin-binding LIM protein 1 protein (174-319AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-133269 / sc-133270
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,HEK293 whole cell lysate,Mouse heart tissueAll lanes: ABLIM1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88,82,49,47,53,85 kDaObserved band size: 88 kDa)

Western Blot (WB) (Western BlotPositive WB detected in:Hela whole cell lysate,HEK293 whole cell lysate,Mouse heart tissueAll lanes: ABLIM1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88,82,49,47,53,85 kDaObserved band size: 88 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7047588 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human heart tissue using MBS7047588 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HepG2 cells using MBS7047588 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS7047588 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-ABLIM1 antibody
May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,559 Da
NCBI Official Full Name
actin-binding LIM protein 1 isoform b
NCBI Official Synonym Full Names
actin binding LIM protein 1
NCBI Official Symbol
ABLIM1
NCBI Official Synonym Symbols
ABLIM; LIMAB1; LIMATIN; abLIM-1
NCBI Protein Information
actin-binding LIM protein 1
UniProt Protein Name
Actin-binding LIM protein 1
Protein Family
UniProt Gene Name
ABLIM1
UniProt Synonym Gene Names
ABLIM; KIAA0059; LIMAB1; abLIM-1
UniProt Entry Name
ABLM1_HUMAN

NCBI Description

This gene encodes a cytoskeletal LIM protein that binds to actin filaments via a domain that is homologous to erythrocyte dematin. LIM domains, found in over 60 proteins, play key roles in the regulation of developmental pathways. LIM domains also function as protein-binding interfaces, mediating specific protein-protein interactions. The protein encoded by this gene could mediate such interactions between actin filaments and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

abLIM: a cytoskeletal LIM protein consisting of a C-terminal cytoskeletal domain fused to an N-terminal domain of four double zinc finger motifs. The C-terminal domain is 50% identical to dematin, an actin-bundling protein of the erythroid cytoskeleton. Undergoes extensive phosphorylation in light-adapted retinas in vivo and its developmental expression in the retina coincides with the elaboration of photoreceptor inner and outer segments. Three distinct splice variant isoforms have been described: ABLIM-l, ABLIM-m, ABLIM-s.

Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: actin cytoskeleton; lamellipodium; stress fiber

Molecular Function: protein binding

Biological Process: lamellipodium biogenesis; organ morphogenesis; visual perception

Research Articles on ABLIM1

Similar Products

Product Notes

The ABLIM1 ablim1 (Catalog #AAA7047588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABLIM1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ABLIM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 1000-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the ABLIM1 ablim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABLIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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