Immunohistochemistry (IHC)
(MBS9609462 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse ABLIM1 Polyclonal Antibody | anti-ABLIM1 antibody
ABLIM1 Antibody
Gene Names
ABLIM1; ABLIM; LIMAB1; LIMATIN; abLIM-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ABLIM1; Polyclonal Antibody; ABLIM1 Antibody; ABLIM 1; ABLIM; abLIM-1; ABLIM-L; ABLIM-M; ABLIM-S; ABLM1_HUMAN; Actin binding double zinc finger protein; Actin binding LIM protein 1; Actin binding LIM protein family member 1; Actin-binding double zinc finger protein; Actin-binding LIM protein 1; Actin-binding LIM protein family member 1; DKFZp781D0148; FLJ14564; KIAA0059; LIM actin binding protein 1; LIMAB 1; LIMAB1; Limatin; MGC1224; MGC125264; OTTHUMP00000020530; OTTHUMP00000020531; OTTHUMP00000020532; OTTHUMP00000020533; OTTHUMP00000020534; OTTHUMP00000020535; OTTHUMP00000020536; OTTHUMP00000020537; RGD1565768; RP11-317F20.1; anti-ABLIM1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ABLIM1 antibody detects endogenous levels of total ABLIM1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
718
Applicable Applications for anti-ABLIM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human ABLIM1
Subcellular Location
Cytoplasm. Cytoplasm > Cytoskeleton. Associated with the cytoskeleton.
Tissue Specificity
Detected in liver, heart, skeletal muscle, brain and retina, where it is concentrated in the inner segment and in the outer plexiform layers.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9609462 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609462 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of ABLIM1 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of ABLIM1 using K562 whole cell lysates)
Related Product Information for anti-ABLIM1 antibody
Function: May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Subunit Structure: Binds F-actin. Interacts with ABRA.
Subunit Structure: Binds F-actin. Interacts with ABRA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 88 kDa
Predicted: 88 kDa
Predicted: 88 kDa
NCBI Official Full Name
actin-binding LIM protein 1 isoform b
NCBI Official Synonym Full Names
actin binding LIM protein 1
NCBI Official Symbol
ABLIM1
NCBI Official Synonym Symbols
ABLIM; LIMAB1; LIMATIN; abLIM-1
NCBI Protein Information
actin-binding LIM protein 1
UniProt Protein Name
Actin-binding LIM protein 1
Protein Family
UniProt Gene Name
ABLIM1
UniProt Synonym Gene Names
ABLIM; KIAA0059; LIMAB1; abLIM-1
NCBI Description
This gene encodes a LIM zinc-binding domain-containing protein that binds to actin filaments and mediates interactions between actin and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2017]
Uniprot Description
May act as scaffold protein (). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Research Articles on ABLIM1
Similar Products
Product Notes
The ABLIM1 ablim1 (Catalog #AAA9609462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABLIM1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ABLIM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the ABLIM1 ablim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABLIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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