Human selectin L ELISA Kit | SELL elisa kit

Human L-Selectin ELISA Kit

Cat. Num. AAA704524

• Gene Names: SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• Reactivity: Human
• Synonyms: selectin L; Human L-Selectin ELISA Kit; Human L-Selectin ELISA kit; CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; lymph node homing; SELL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human L-selectin. No significant cross-reactivity or interference between human L-selectin and analogues was observed.
• Sequence Length: 385

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml.
• Sensitivity: The minimum detectable dose of human L-selectin is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELL elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for L-selectin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any L-selectin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L-selectin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L-selectin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 262206315
• NCBI GeneID: 6402
• NCBI Accession #: NP_000646.2
• NCBI GenBank Nucleotide #: NM_000655.4
• UniProt Accession #: P14151; P15023; Q9UJ43; B2R6Q8
• Molecular Weight: 42,187 Da
• NCBI Official Full Name: L-selectin
• NCBI Official Synonym Full Names: selectin L
• NCBI Official Symbol: SELL
• NCBI Official Synonym Symbols: TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• NCBI Protein Information: L-selectin; gp90-MEL; pln homing receptor; lymph node homing receptor; lymphocyte adhesion molecule 1; leukocyte surface antigen Leu-8; CD62 antigen-like family member L; leukocyte-endothelial cell adhesion molecule 1
• UniProt Protein Name: L-selectin
• Protein Family: L-selectin
• UniProt Gene Name: SELL
• UniProt Synonym Gene Names: LNHR; LYAM1; LAM-1; LECAM1
• UniProt Entry Name: LYAM1_HUMAN

NCBI Description

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.1 PublicationManual assertion based on experiment in:Ref.12