Human TIMP metallopeptidase inhibitor 1 ELISA Kit | TIMP1 elisa kit

Human Tissue Inhibitor Of Matrix Metalloprotease-1, TIMP-1 ELISA Kit

Cat. Num. AAA704361

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP
• Reactivity: Human
• Synonyms: TIMP metallopeptidase inhibitor 1; Human Tissue Inhibitor Of Matrix Metalloprotease-1; TIMP-1 ELISA Kit; Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinase 1; Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1); TIMP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TIMP1. No significant cross-reactivity or interference between Human TIMP1 and analogues was observed.
• Sequence Length: 207

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 0.39 ng/ml-25 ng/ml
• Sensitivity: 0.434 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cardiovascular
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP1 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TIMP-1 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated TIMP-1 and incubated. A competitive inhibition reaction is launched between TIMP-1 (Standards or samples) and HRP-conjugated TIMP-1 with the pre-coated antibody specific for TIMP-1. The more amount of TIMP-1 in samples, the less antibody bound by HRP-conjugated TIMP-1. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TIMP-1 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507509
• NCBI GeneID: 7076
• NCBI Accession #: NP_003245.1
• NCBI GenBank Nucleotide #: NM_003254.2
• UniProt Accession #: P01033; Q14252; Q9UCU1
• Molecular Weight: 23,171 Da
• NCBI Official Full Name: metalloproteinase inhibitor 1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP
• NCBI Protein Information: metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
• UniProt Entry Name: TIMP1_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.12 PublicationsManual assertion based on experiment in:Ref.1