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Typical Testing Data/Standard Curve (for reference only)

Human phospholipid transfer protein ELISA Kit | PLTP elisa kit

Human phospholipid transfer protein, PLTP ELISA Kit

Gene Names
PLTP; BPIFE; HDLCQ9
Reactivity
Human
Synonyms
phospholipid transfer protein; Human phospholipid transfer protein; PLTP ELISA Kit; Human phospholipid transfer protein (PLTP) Elisa Kit; HDLCQ9; lipid transfer protein II; PLTP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PLTP. No significant cross-reactivity or interference between Human PLTP and analogues was observed.
Sequence Length
398
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
2.34 ng/ml-150 ng/ml
Sensitivity
0.58 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transport
Protein Biological Process 3
Lipid transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLTP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLTP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLTP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLTP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLTP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,739 Da
NCBI Official Full Name
phospholipid transfer protein isoform c
NCBI Official Synonym Full Names
phospholipid transfer protein
NCBI Official Symbol
PLTP
NCBI Official Synonym Symbols
BPIFE; HDLCQ9
NCBI Protein Information
phospholipid transfer protein; lipid transfer protein II; BPI fold containing family E
UniProt Protein Name
Phospholipid transfer protein
UniProt Gene Name
PLTP
UniProt Entry Name
PLTP_HUMAN

NCBI Description

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.

Research Articles on PLTP

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Product Notes

The Human PLTP pltp (Catalog #AAA703855) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703855 ELISA Kit recognizes Human PLTP. It is sometimes possible for the material contained within the vial of "phospholipid transfer protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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