Human Pancreatic Polypeptide, PP ELISA Kit | PP elisa kit

Human Pancreatic Polypeptide, PP ELISA Kit

Cat. Num. AAA702573

• Gene Names: PPY; PNP
• Reactivity: Human
• Synonyms: Pancreatic Polypeptide; PP; Human Pancreatic Polypeptide; PP ELISA Kit; PP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PP. No significant cross-reactivity or interference between human PP and analogues was observed.
• Sequence Length: 95

• Samples: Serum, plasma
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of human PP is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PP elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 119572034
• NCBI GeneID: 5539
• UniProt Accession #: P01298
• Molecular Weight: 10,445 Da
• NCBI Official Full Name: pancreatic polypeptide
• NCBI Official Synonym Full Names: pancreatic polypeptide
• NCBI Official Symbol: PPY
• NCBI Official Synonym Symbols: PNP
• NCBI Protein Information: pancreatic prohormone; PP; pancreatic polypeptide Y; prepro-PP (prepropancreatic polypeptide)
• UniProt Protein Name: Pancreatic prohormone
• UniProt Gene Name: PPY
• UniProt Synonym Gene Names: PNP; PP; PH; PI
• UniProt Entry Name: PAHO_HUMAN

NCBI Description

This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.