Human angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit | AGT elisa kit

Human angiotensinogen, aGT ELISA Kit

Cat. Num. AAA702389

• Gene Names: AGT; ANHU; SERPINA8
• Reactivity: Human
• Synonyms: angiotensinogen (serpin peptidase inhibitor; clade A; member 8); Human angiotensinogen; aGT ELISA Kit; Human angiotensinogen (aGT) ELISA Kit; ANHU; FLJ92595; FLJ97926; SERPINA8; alpha-1 antiproteinase; antitrypsin; angiotensin I; angiotensin II; angiotensinogen; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; AGT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human aGT. No significant cross-reactivity or interference between human aGT and analogues was observed.
• Sequence Length: 485

• Samples: Serum, plasma, urine, cell culture supernates
• Assay Type: Sandwich
• Detection Range: 78 pg/ml -5000 pg/ml.
• Sensitivity: The minimum detectable dose of human aGT is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGT elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for aGT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any aGT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for aGT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of aGT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557287
• NCBI GeneID: 183
• NCBI Accession #: NP_000020.1
• NCBI GenBank Nucleotide #: NM_000029.3
• UniProt Accession #: P01019; Q16358; Q16359; Q96F91
• Molecular Weight: 53,154 Da
• NCBI Official Full Name: angiotensinogen preproprotein
• NCBI Official Synonym Full Names: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• NCBI Official Symbol: AGT
• NCBI Official Synonym Symbols: ANHU; SERPINA8
• NCBI Protein Information: angiotensinogen; serpin A8; angiotensin I; angiotensin II; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor
• UniProt Protein Name: Angiotensinogen
• Protein Family: Angiotensinogen
• UniProt Gene Name: AGT
• UniProt Synonym Gene Names: SERPINA8; Ang I; Ang II; Ang III; Ang IV
• UniProt Entry Name: ANGT_HUMAN

NCBI Description

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.3 PublicationsManual assertion based on experiment in:Ref.11