Rat Laminin, LN ELISA Kit | LN elisa kit

Rat Laminin, LN ELISA Kit

Cat. Num. AAA702171

• Gene Names: LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
• Reactivity: Rat
• Synonyms: Laminin; LN; Rat Laminin; LN ELISA Kit; LN ELISA kit; LN elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat LN. No significant cross-reactivity or interference between rat LN and analogues was observed.
• Sequence Length: 1111

• Samples: Serum, plasma
• Assay Type: Competitive
• Detection Range: 62.5 pg/ml -4000 pg/ml.
• Sensitivity: The minimum detectable dose of rat LN is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LN elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LN. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LN. The competitive inhibition reaction is launched between with pre-coated LN and LN in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LN in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 34232
• NCBI GeneID: 3918
• UniProt Accession #: Q13753; Q02536; Q02537; Q13752; Q14941; Q14DF7; Q5VYE8
• Molecular Weight: 121,604 Da
• NCBI Official Full Name: Laminin
• NCBI Official Synonym Full Names: laminin, gamma 2
• NCBI Official Symbol: LAMC2
• NCBI Official Synonym Symbols: B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
• NCBI Protein Information: laminin subunit gamma-2; BM600-100kDa; laminin B2t chain; CSF 140 kDa subunit; nicein subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; epiligrin subunit gamma; cell-scattering factor 140 kDa subunit; large adhesive scatter factor 140 kDa subunit
• UniProt Protein Name: Laminin subunit gamma-2
• UniProt Gene Name: LAMC2
• UniProt Synonym Gene Names: LAMB2T; LAMNB2; CSF 140 kDa subunit
• UniProt Entry Name: LAMC2_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.1 PublicationManual assertion based on experiment in:Ref.7