Human retinol binding protein 4, plasma ELISA Kit | RBP4 elisa kit

Human Retinol binding protein 4, RBP-4 ELISA Kit

Cat. Num. AAA701969

• Gene Names: RBP4; RDCCAS
• Reactivity: Human
• Synonyms: retinol binding protein 4; plasma; Human Retinol binding protein 4; RBP-4 ELISA Kit; OTTHUMP00000020116; retinol-binding protein 4; interstitial; RBP4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human RBP4. No significant cross-reactivity or interference between Human RBP4 and analogues was observed.
• Sequence Length: 201

• Samples: Serum, plasma, urine, cell culture supernates, tissue homogenates
• Assay Type: Competitive
• Detection Range: 0.25 ug/ml-100 ug/ml
• Sensitivity: 0.1 ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Neurobiology
• Protein Biological Process 3: Sensory transduction
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP4 elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to RBP-4 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated RBP-4 and incubated. A competitive inhibition reaction is launched between RBP-4 (Standards or samples) and HRP-conjugated RBP-4 with the pre-coated antibody specific for RBP-4. The more amount of RBP-4 in samples, the less antibody bound by HRP-conjugated RBP-4. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of RBP-4 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 55743122
• NCBI GeneID: 5950
• NCBI Accession #: NP_006735.2
• NCBI GenBank Nucleotide #: NM_006744.3
• UniProt Accession #: P02753; O43478; O43479; Q5VY24; Q8WWA3; Q9P178; D3DR38
• Molecular Weight: 23,010 Da
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS
• NCBI Protein Information: retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol-binding protein 4, interstitial
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRO2222; PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.