Human complement factor D (adipsin) ELISA Kit | CFD elisa kit

Human Adipsin ELISA Kit

Cat. Num. AAA701871

• Gene Names: CFD; DF; ADN; PFD; ADIPSIN
• Reactivity: Human
• Synonyms: complement factor D (adipsin); Human Adipsin ELISA Kit; ADIPSIN; ADN; DF; PFD; C3 convertase activator; D component of complement (adipsin) ; complement factor D; complement factor D preproprotein; properdin factor D; CFD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human adipsin. No significant cross-reactivity or interference between human adipsin and analogues was observed.
• Sequence Length: 253

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFD elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for adipsin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any adipsin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for adipsin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of adipsin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 42544239
• NCBI GeneID: 1675
• NCBI Accession #: NP_001919.2
• NCBI GenBank Nucleotide #: NM_001928.2
• UniProt Accession #: P00746; Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4; B4DV76
• Molecular Weight: 27,033 Da
• NCBI Official Full Name: complement factor D preproprotein
• NCBI Official Synonym Full Names: complement factor D (adipsin)
• NCBI Official Symbol: CFD
• NCBI Official Synonym Symbols: DF; ADN; PFD; ADIPSIN
• NCBI Protein Information: complement factor D; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin)
• UniProt Protein Name: Complement factor D
• Protein Family: Complement factor
• UniProt Gene Name: CFD
• UniProt Synonym Gene Names: DF; PFD
• UniProt Entry Name: CFAD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Catalytic activity: Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.