Chicken Lysozyme C (LZM) ELISA Kit | LYZ elisa kit

Chicken Lysozyme C, LZM ELISA Kit

Cat. Num. AAA701562

• Gene Names: LYZ; LZM
• Reactivity: Chicken
• Synonyms: Lysozyme C (LZM); Chicken Lysozyme C; LZM ELISA Kit; Chicken Lysozyme C (LYZ) ELISA kit; LZM; lysozyme; 1; 4-beta-N-acetylmuramidase C; lysozyme C; Chicken Lysozyme C (LYZ); LYZ elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of chicken lysozyme. No significant cross- reactivity or interference between chicken lysozyme and analogues was observed.
• Sequence Length: 148

• Samples: Serum, plasma and egg white
• Assay Type: Quantitative Competitive
• Detection Range: 15.625 ng/ml-4000 ng/ml
• Sensitivity: <14.876 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LYZ elisa kit

Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for lysozyme has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated lysozyme. A competitive inhibition reaction is launched between lysozyme (Standards or samples) and biotin-conjugated lysozyme with the pre-coated lysozyme antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of lysozyme bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 307142
• NCBI GeneID: 4069
• UniProt Accession #: P61626; P00695; Q13170; Q9UCF8
• Molecular Weight: 16,537 Da
• NCBI Official Full Name: lysozyme (EC 3.2.1.17)
• NCBI Official Synonym Full Names: lysozyme
• NCBI Official Symbol: LYZ
• NCBI Official Synonym Symbols: LZM
• NCBI Protein Information: lysozyme C; c-type lysozyme; 1,4-beta-N-acetylmuramidase C
• UniProt Protein Name: Lysozyme C
• UniProt Gene Name: LYZ
• UniProt Synonym Gene Names: LZM
• UniProt Entry Name: LYSC_HUMAN

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the anti-microbial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. Missense mutations in LYZ have been identified in heritable renal amyloidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

Catalytic activity: Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.