Rabbit anti-Human Transcription intermediary factor 1-beta Polyclonal Antibody | anti-TRIM28 antibody
Rabbit anti-human Transcription intermediary factor 1-beta polyclonal Antibody
Western Blot (WB)
(Western BlotPositive WB detected in: PC3 whole cell lysate, MCF-7 whole cell lysate,K562 whole cell lysateAll lanes: TRIM28 antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89,80 KDaObserved band size: 89 KDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using MBS7006602 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using MBS7006602 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating TRIM28 in Hela whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS7006602 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7006602 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
This protein mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Uniprot Description
TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Protein type: Transcription, coactivator/corepressor; Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Nuclear receptor co-regulator; Kinase, protein; Protein kinase, atypical; Ubiquitin ligase; ATYPICAL group; TIF1 family
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: nuclear heterochromatin; nucleoplasm; nucleus
Molecular Function: DNA binding; ligase activity; protein binding; protein kinase activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: convergent extension involved in axis elongation; DNA methylation during embryonic development; DNA repair; embryo implantation; epithelial to mesenchymal transition; gene expression; innate immune response; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of DNA repair; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein oligomerization; protein sumoylation; protein ubiquitination; transcription initiation from RNA polymerase II promoter