Typical Testing Data/Standard Curve (for reference only)
Human aquaporin 1 (Colton blood group) ELISA Kit | AQP1 elisa kit
Human Aquaporin 1, AQP-1 ELISA Kit
Gene Names
AQP1; CO; CHIP28; AQP-CHIP
Reactivity
Human
Synonyms
aquaporin 1 (Colton blood group); Human Aquaporin 1; AQP-1 ELISA Kit; AQP-CHIP; CHIP28; CO; MGC26324; Colton blood group; aquaporin 1; aquaporin 1 (channel-forming integral protein; 28kDa; CO blood group) ; channel-like integral membrane protein; 28-kDa; AQP1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human AQP1. No significant cross-reactivity or interference between Human AQP1 and analogues was observed.
Sequence Length
186
Samples
Serum, plasma, cell culture supernates, Cell lysates
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transport
Protein Biological Process 3
Transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AQP1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique.Antibody specific for AQP-1 has been pre-coated onto a microplate. Standardsand samples are pipetted into the wells and any AQP-1 present is bound by theimmobilized antibody. After removing any unbound substances, abiotin-conjugated antibody specific for AQP-1 is added to the wells. Afterwashing, avidin conjugated Horseradish Peroxidase (HRP) is added to thewells. Following a wash to remove any unbound avidin-enzyme reagent, asubstrate solution is added to the wells and color develops in proportion to theamount of AQP-1 bound in the initial step. The color development is stoppedand the intensity of the color is measured.
References
Aqueous Humor Biomarkers of Müller Cell Activation in Diabetic Eyes. Stela Vujosevic; Alessandra Micera; Silvia Bini; Marianna Berton; Graziana Esposito; Edoardo Midena (2015)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,526 Da
NCBI Official Full Name
aquaporin-1 isoform 2
NCBI Official Synonym Full Names
aquaporin 1 (Colton blood group)
NCBI Official Symbol
AQP1
NCBI Official Synonym Symbols
CO; CHIP28; AQP-CHIP
NCBI Protein Information
aquaporin-1; aquaporin-CHIP; urine water channel; channel-like integral membrane protein, 28-kDa; water channel protein for red blood cells and kidney proximal tubule; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group)
UniProt Protein Name
Aquaporin-1
UniProt Gene Name
AQP1
UniProt Synonym Gene Names
CHIP28; AQP-1
UniProt Entry Name
AQP1_HUMAN
NCBI Description
Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
Function: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.1 PublicationManual assertion based on experiment in:Ref.13
Research Articles on AQP1
Similar Products
Product Notes
The Human AQP1 aqp1 (Catalog #AAA700396) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700396 ELISA Kit recognizes Human AQP1. It is sometimes possible for the material contained within the vial of "aquaporin 1 (Colton blood group), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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