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Western Blot (WB) (Western blot analysis of HOXA9 Antibody (C-term) in A2058 cell line lysates (35ug/lane). HOXA9 (arrow) was detected using the purified Pab.)

Rabbit anti-Human HOXA9 Polyclonal Antibody | anti-HOXA9 antibody

HOXA9, CT (HOXA9, HOX1G, Homeobox protein Hox-A9, Homeobox protein Hox-1G) (PE)

Gene Names
HOXA9; HOX1; ABD-B; HOX1G; HOX1.7
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
HOXA9; Polyclonal Antibody; CT (HOXA9; HOX1G; Homeobox protein Hox-A9; Homeobox protein Hox-1G) (PE); Homeobox protein Hox-1G; anti-HOXA9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human HOXA9
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-HOXA9 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
HOXA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the C-terminal region of human HOXA9.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HOXA9 Antibody (C-term) in A2058 cell line lysates (35ug/lane). HOXA9 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of HOXA9 Antibody (C-term) in A2058 cell line lysates (35ug/lane). HOXA9 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(HOXA9 Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (HOXA9 Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-HOXA9 antibody
HOXA9 is the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development.
Product Categories/Family for anti-HOXA9 antibody
References
Scott, M.P. Cell 71 (4), 551-553 (1992).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
272
NCBI Official Full Name
Homeobox protein Hox-A9
NCBI Official Synonym Full Names
homeobox A9
NCBI Official Symbol
HOXA9
NCBI Official Synonym Symbols
HOX1; ABD-B; HOX1G; HOX1.7
NCBI Protein Information
homeobox protein Hox-A9; homeobox protein Hox-1G; homeodomain protein HOXA9
UniProt Protein Name
Homeobox protein Hox-A9
Protein Family
UniProt Gene Name
HOXA9
UniProt Synonym Gene Names
HOX1G
UniProt Entry Name
HXA9_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Read-through transcription exists between this gene and the upstream homeobox A10 (HOXA10) gene.[provided by RefSeq, Mar 2011]

Uniprot Description

HOXA9: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98. A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2. Belongs to the Abd-B homeobox family.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; sequence-specific DNA binding

Biological Process: embryonic forelimb morphogenesis; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; multicellular organismal development; embryonic skeletal development; male gonad development; uterus development; endothelial cell activation; anterior/posterior pattern formation; regulation of transcription, DNA-dependent; mammary gland development; single fertilization; spermatogenesis; proximal/distal pattern formation

Research Articles on HOXA9

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Product Notes

The HOXA9 hoxa9 (Catalog #AAA6307780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HOXA9, CT (HOXA9, HOX1G, Homeobox protein Hox-A9, Homeobox protein Hox-1G) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOXA9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HOXA9 hoxa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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