Rabbit anti-Human Poly (A)-specific ribonuclease PARN Polyclonal Antibody | anti-PARN antibody

Rabbit anti-human Poly(A)-specific ribonuclease PARN polyclonal Antibody

Cat. Num. AAA7000840

• Gene Names: PARN; DAN; DKCB6; PFBMFT4
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Poly (A)-specific ribonuclease PARN; Polyclonal Antibody; Rabbit anti-human Poly(A)-specific ribonuclease PARN polyclonal Antibody; PAM16 MAGMAS; TIM16; TIMM16 CGI-136; anti-PARN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 578

• Applicable Applications for anti-PARN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
• Immunogen: Recombinant human Poly(A)-specific ribonuclease PARN protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-47619 / sc-47618 / sc-135242
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: Poly(A)-specific ribonuclease PARN antibody at 2ug/mlLane 1:Jurkat whole cell lysateLane 2:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 74,67,68,53 kDaObserved band size: 73 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embeded human prostate using MBS7000840 at dilution of 1:50)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7000840 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PARN antibody

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.

This protein is a 3 -exoribonuclease, with similarity to the RNase D family of 3 -exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PARN antibody

Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 197333695
• NCBI GeneID: 5073
• NCBI Accession #: NP_001127949.1
• NCBI GenBank Nucleotide #: NM_001134477.2
• UniProt Accession #: O95453; B2RCB3; B4DDG8; B4DSB0; B4DWR4; B4E1H6
• Molecular Weight: 52,989 Da
• NCBI Official Full Name: poly(A)-specific ribonuclease PARN isoform 2
• NCBI Official Synonym Full Names: poly(A)-specific ribonuclease
• NCBI Official Symbol: PARN
• NCBI Official Synonym Symbols: DAN; DKCB6; PFBMFT4
• NCBI Protein Information: poly(A)-specific ribonuclease PARN
• UniProt Protein Name: Poly(A)-specific ribonuclease PARN
• Protein Family: Poly(A)-specific ribonuclease
• UniProt Gene Name: PARN
• UniProt Synonym Gene Names: DAN
• UniProt Entry Name: PARN_HUMAN

NCBI Description

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

PARN: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Ubiquitous. Belongs to the CAF1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; EC 3.1.13.4; Ribonuclease; Nucleolus

Chromosomal Location of Human Ortholog: 16p13

Cellular Component: cytoplasm; cytosol; nucleolus; nucleus

Molecular Function: metal ion binding; mRNA 3'-UTR binding; nuclease activity; nucleotide binding; poly(A)-specific ribonuclease activity; protein binding; protein kinase binding

Biological Process: female gamete generation; gene expression; mRNA catabolic process, deadenylation-dependent decay; mRNA catabolic process, nonsense-mediated decay; poly(A) tail shortening; regulation of mRNA stability; RNA modification

Disease: Dyskeratosis Congenita, Autosomal Recessive, 6; Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 4