MIG active protein

Human MIG (CXCL9)

Cat. Num. AAA695824

• Gene Names: CXCL9; CMK; MIG; Humig; SCYB9; crg-10
• Reactivity: Species Reactivity: Human
• Purity: >98% by SDS-PAGE gel and HPLC analyses.
• Synonyms: MIG; Human MIG (CXCL9); Recombinant Human MIG (CXCL9); MIG active protein

• Host: E Coli
• Reactivity: Species Reactivity: Human
• Purity/Purification: >98% by SDS-PAGE gel and HPLC analyses.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
• Sequence: TPVVRKGRC SCISTNQGT IHLQSLKDL KQFAPSPSC EKIEIIATL KNGVQTCLN PDSADVKEL IKKWEKQVS QKKKQKNGK KHQKKKVLK VRKSQRSRQ KKTT

• Biological Activity: Determined by its ability to chemoattract human peripheral blood T lymphocytes using a concentration range of 10.0-100.0 ng/ml.
• Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable).
• Cell Type: Monocytes and Macrophages
• Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug).
• Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: Store at -20°C.

Testing Data

Related Product Information for MIG active protein

MIG, a CXC chemokine, is produced by IFN stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells. Recombinant human MIG is an 11.7 kDa protein containing 103 amino acid residues, including the four highly conserved cysteine residues present in CXC chemokines.

Product Categories/Family for MIG active protein

Innate Immunity; Angiogenesis; Inflammation; Chemotaxis

NCBI and Uniprot Product Information

• NCBI GI #: 585487
• NCBI GeneID: 4283
• UniProt Accession #: Q07325; Q503B4
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Official Synonym Symbols: CMK; MIG; Humig; SCYB9; crg-10
• NCBI Protein Information: C-X-C motif chemokine 9; small-inducible cytokine B9; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: Migration and invasion enhancer
• UniProt Gene Name: CXCL9
• UniProt Synonym Gene Names: CMK; MIG; SCYB9; HuMIG; MIG
• UniProt Entry Name: CXCL9_HUMAN

NCBI Description

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; defense response; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; positive regulation of myoblast differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; defense response to virus