Dkk-2 active protein

Human Dkk-2

Cat. Num. AAA692245

• Gene Names: DKK2; DKK-2
• Reactivity: Human
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Synonyms: Dkk-2; Human Dkk-2; Dickkopf-related protein-2; Dickkopf-2; Dkk-2 active protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: SQIGSSRAKL NSIKSSLGGE TPGQAANRSA GMYQGLAFGG SKKGKN LGQA YPCSSDKECE VGRYCHSPHQ GSSACMVCRR KKKRCHRDGM C CPSTRCNNG ICIPVTESIL TPHIPALDGT RHRDRNHGHY SNHDLGW QNL GRPHTKMSHI KGHEGDPCLR SSDCIEGFCC ARHFWTKICK PV LHQGEVCT KQRKKGSHGL EIFQRCDCAK GLSCKVWKDA TYSSKARL HV CQKI
• Sequence Length: 259

• Biological Activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 0.5 - 1.0 ug/ml.

Related Product Information for Dkk-2 active protein

The dickkopf (DKK)-related protein family is comprised of four central members, DKK-1 - 4, along with the distantly-related DKK family member DKK-11 (Soggy), which is thought to be a descendent of an ancestral DKK-3 precursor due to its unique sequence homology to DKK-3 and no other DKK family member. DKK family members, with the exception of the divergent Soggy, share two conserved cysteine-rich domains and show very little sequence similarity outside of these domains. Playing an important regulatory role in vertebrate development through localized inhibition of Wnt-regulated processes, including anterior-posterior axial patterning, limb development, somitogenesis, and eye formation, DKKs have also been implicated post-developmentally in bone formation, bone disease, cancer, and neurodegenerative diseases. DKK proteins typically play an important regulatory role in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. DKK-2 has been shown to both inhibit and enhance canonical Wnt signaling; enhancing Wnt signaling through direct high-affinity binding of DKK-2 to LRP6 during LRP6 overexpression, while inhibiting Wnt signaling and promoting LRP6 internalization through the formation of a ternary complex between DKK-2, LRP6, and Kremen-2. Recombinant Human DKK-2 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 25.8 kDa and contains 234 amino acid residues. Due to glycosylation, human DKK-2 migrates at an apparent molecular weight of approximately 31-36 kDa by SDS-PAGE analysis under non-reducing conditions.

Product Categories/Family for Dkk-2 active protein

Recombinant Protein; Cytokines & Growth Factors; Dkk-2

NCBI and Uniprot Product Information

• NCBI GI #: 7657023
• NCBI GeneID: 27123
• NCBI Accession #: NP_055236.1
• NCBI GenBank Nucleotide #: NM_014421.2
• UniProt Accession #: Q9UBU2; Q9UIU3; A0AVE9; B2R6S7
• Molecular Weight: 28,447 Da
• NCBI Official Full Name: dickkopf-related protein 2
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 2
• NCBI Official Symbol: DKK2
• NCBI Official Synonym Symbols: DKK-2
• NCBI Protein Information: dickkopf-related protein 2
• UniProt Protein Name: Dickkopf-related protein 2
• UniProt Gene Name: DKK2
• UniProt Synonym Gene Names: Dickkopf-2; Dkk-2; hDkk-2
• UniProt Entry Name: DKK2_HUMAN

Uniprot Description

DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space