ECGFpro1 supplement
Bovine ECGFpro1 (complete for blood ECs)
Reactivity
Human Cells, Mouse Cells, Cattle Cells
Synonyms
ECGFpro1; Bovine ECGFpro1 (complete for blood ECs); Endothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS); ECGFpro1 supplement
Host
Cattle
Reactivity
Human Cells, Mouse Cells, Cattle Cells
Form/Format
Crude extract; Lyophilized (freeze-dried)
Species
Bovine
Reconstitution
Reconstitute the contents of the vial in 2 ml of prewarmed (37 degree C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.
Biological Activity
Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 ug/ml, optimal concentration with heparin (50 ug/ml) is about 12 ug/ml.
Remarks
BSE-free tested!
Preparation and Storage
Also stable at 4 degree C for several weeks it is recommended to store the product below 0 degree C. After reconstitution the product shout be stored in aliquots at -20 degree C to -70 degree C.
Related Product Information for ECGFpro1 supplement
Endothelila cell growth factor (ECGF) or endothelial cell growth supplement (ECGS) is the term used also for a bovine (or porcine) brain extract containing activities promoting growth and maintenance of endothelial cells (T. Maciag, 1972 and 1982). In the early days this extract has employed as a cell culture supplement together with fetal calf serum to promote long-term serial propagation of human and animla endothelial cells. Its use can reduce the requirement for serum, and heparin has been shown to potentiate the mitogenic activity of the extract (PB Gordon, 1983; Glassberg et al., 1982). The preparation can be used also to grow hybridoma cells without feeder cells (Pintus, 1983). The active factors in these brain extracts have been identified as variants of aFGF (Burgesse, 1985). This ECGF product is supplemented with human rec. VEGF-A in physiological concentrations in order to support growth and differentiation of blood endothelial cells in culture.
Product Categories/Family for ECGFpro1 supplement
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Product Notes
The ECGFpro1 (Catalog #AAA692241) is a Supplement produced from Cattle and is intended for research purposes only. The product is available for immediate purchase. The Bovine ECGFpro1 (complete for blood ECs) reacts with Human Cells, Mouse Cells, Cattle Cells and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "ECGFpro1, Supplement" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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