IL-12 active protein
Mouse IL-12
Gene Names
Il12a; p35; Ll12a; Il-12a; IL-12p35
Reactivity
Mouse
Purity
> 95% (SDS-PAGE; HPLC)
Synonyms
IL-12; Mouse IL-12; Recombinant Murine IL-12; IL-12 active protein
Host
CHO Cells
Reactivity
Mouse
Purity/Purification
> 95% (SDS-PAGE; HPLC)
Form/Format
Lyophilized
Sequence
p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLK HYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQ QIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTIT VKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN K TFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGM ASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQ QNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWS TP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQC KGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Sequence Length
236
Endotoxin Level
< 0.1 ng/ug of IL-12
Biological Activity
Assay #1: The ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml (> 1 x 107 units/mg). Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is <=0.1ng/ml (>=1 x 107 units/mg).
Related Product Information for IL-12 active protein
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Product Categories/Family for IL-12 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75 kDa
NCBI Official Full Name
interleukin-12 subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 12a
NCBI Official Symbol
Il12a
NCBI Official Synonym Symbols
p35; Ll12a; Il-12a; IL-12p35
NCBI Protein Information
interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit
UniProt Protein Name
Interleukin-12 subunit alpha
UniProt Gene Name
Il12a
UniProt Synonym Gene Names
IL-12A; CLMF p35
UniProt Entry Name
IL12A_MOUSE
Uniprot Description
Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC
By similarity.
Subunit structure: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-6 superfamily.
Research Articles on IL-12
Similar Products
Product Notes
The IL-12 il12a (Catalog #AAA692163) is an Active Protein produced from CHO Cells and is intended for research purposes only. The product is available for immediate purchase. The Mouse IL-12 reacts with Mouse and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLK HYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQ QIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTIT VKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN K TFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGM ASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQ QNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWS TP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQC KGGNVCV QAQDRYYNSS CSKWACVPCR VRS. It is sometimes possible for the material contained within the vial of "IL-12, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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