Visfatin Active Protein | NAMPT active protein

Human Visfatin

Cat. Num. AAA692109

• Gene Names: NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• Reactivity: Human
• Purity: 98 % (SDS-PAGE, HPLC)
• Synonyms: Visfatin; Human Visfatin; Recombinant Human Visfatin; NAMPT active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: 98 % (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLK GKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE I KAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVA TNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IG ASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAW GK DHE kDaFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RH LIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKL LP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQ KLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHR T PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKN AQLNIE LEAAHH
• Sequence Length: 491

• Endotoxin Level: < 0.1 ng per ug of Visfatin
• Biological Activity: The ED50 was determined by the dose-dependant proliferation of RPMI 8226 cells. The expected ED50 for this effect is 15.0-20.0 ng/ml.

Related Product Information for NAMPT active protein

Visfatin is a 55 kDa protein produced and secreted primarily by white adipose tissue. Recently, Visfatin was isolated from visceral fat deposits and shown to possess insulin-mimetic activity. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. The binding affinity of Visfatin for the insulin receptor is similar to that of insulin, but it does not compete with insulin, suggesting that the two proteins interact with different receptor sites. The circulating levels of Visfatin are much lower than those of insulin and are not affected by feeding, implying that the hypoglycemic effect of Visfatin may not be of physiological importance. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. Another similarity between Visfatin and Leptin is that their amino acid sequence is highly conserved across different mammalian species and shows no homology to any known protein. Receptors for both Leptin (Ob-R) and Visfatin (i.e. the insulin receptor) are expressed by neurons within the arcuate nucleus of the hypothalamus, a brain area that plays a pivotal role in the regulation of energy metabolism. Although the metabolic function of Visfatin is still unknown, it appears that this newly identified adipocytokine might play an important role, similar to that of Leptin, in the regulation of body weight, i.e. as an afferent signal reflecting excess body fat. Recombinant human Visfatin is a 52.5 kDa protein containing 465 amino acid residues (isoform 1).

Product Categories/Family for NAMPT active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 5031977
• NCBI GeneID: 10135
• NCBI Accession #: NP_005737.1
• NCBI GenBank Nucleotide #: NM_005746.2
• UniProt Accession #: P43490; Q3KQV0; Q8WW95; A4D0Q9; A4D0R0
• Molecular Weight: 52.5 kDa
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: NAMPT
• NCBI Official Synonym Symbols: VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• Protein Family: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: NAMPT
• UniProt Synonym Gene Names: PBEF; PBEF1; NAmPRTase; Nampt
• UniProt Entry Name: NAMPT_HUMAN

NCBI Description

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Uniprot Description

Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Ref.10

Catalytic activity: Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate.

Enzyme regulation: Inhibited by FK866. FK866 competes for the same binding site as nicotinamide, but due to its very low dissociation rate, it is essentially an irreversible inhibitor. Ref.11

Pathway: Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.

Subunit structure: Homodimer. Ref.11

Subcellular location: Nucleus. Cytoplasm

By similarity. Secreted. Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum. Ref.9 Ref.10

Tissue specificity: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Sequence similarities: Belongs to the NAPRTase family.

Sequence caution: The sequence AAQ96862.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAL24400.1 differs from that shown. Reason: Erroneous gene model prediction.