Slit2-N active protein

Human Slit2-N

Cat. Num. AAA691961

• Gene Names: SLIT2; SLIL3; Slit-2
• Reactivity: Human
• Purity: 98 % (SDS-PAGE, HPLC)
• Synonyms: Slit2-N; Human Slit2-N; Recombinant Human Slit2-N; Slit2-N active protein

• Host: HEK 293 Cells
• Reactivity: Human
• Purity/Purification: 98 % (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRIT KTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL Q LFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQ ISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LH SNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKR EF VCSGHQSFMA PSCSVLHCPA ACTCSNNIVD CRGKGLTEIP TNL PETITEI RLEQNTIKVI PPGAFSPYKK LRRIDLSNNQ ISELAPDAF Q GLRSLNSLVL YGNKITELPK SLFEGLFSLQ LLLLNANKIN CLRV DAFQDL HNLNLLSLYD NKLQTIAKGT FSPLRAIQTM HLAQNPFICD CHLKWLADYL HTNPIETSGA RCTSPRRLAN KRIGQIKSKK FRCSA KEQYF IPGTEDYRSK LSGDCFADLA CPEKCRCEGT TVDCSNQKLN KIPEHIPQYT AELRLNNNEF TVLEATGIFK KLPQLRKINF SNNKIT DIEE GAFEGASGVN EILLTSNRLE NVQHKMFKGL ESLKTLMLRS N RITCVGNDS FIGLSSVRLL SLYDNQITTV APGAFDTLHS LSTLNLL ANP FNCNCYLAWL GEWLRKKRIV TGNPRCQKPY FLKEIPIQDV AI QDFTCDDG NDDNSCSPLS RCPTECTCLD TVVRCSNKGL KVLPKGIP RD VTELYLDGNQ FTLVPKELSN YKHLTLIDLS NNRISTLSNQ SFS NMTQLLT LILSYNRLRC IPPRTFDGLK SLRLLSLHGN DISVVPEGA F NDLSALSHLA IGANPLYCDC NMQWLSDWVK SEYKEPGIAR CAGP GEMADK LLLTTPSKKF TCQGPVDVNI LAKCNPCLSN PCKNDGTCNS DPVDFYRCTC PYGFKGQDCD VPIHACISNP CKHGGTCHLK EGEED GFWCI CADGFEGENC EVNVDDCEDN DCENNSTCVD GINNYTCLCP PEYTGELCEE KLDFCAQDLN PCQHDSKCIL TPKGFKCDCT PGYVGE HCDI DFDDCQDNKC KNGAHCTDAV NGYTCICPEG YSGLFCEFSP P MV
• Sequence Length: 1529

• Endotoxin Level: < 0.1 ng per ug of Relaxin-3
• Biological Activity: Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation.

Related Product Information for Slit2-N active protein

Slit2 is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and can also act as a chemo attractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. Slit2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. Slit2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant human Slit2-N is a 1088 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 110.0-116.0 kDa by SDS-PAGE analysis under reducing conditions.

Product Categories/Family for Slit2-N active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4759146
• NCBI GeneID: 9353
• NCBI Accession #: NP_004778.1
• NCBI GenBank Nucleotide #: NM_004787.2
• UniProt Accession #: O94813; O95710; Q17RU3; Q9Y5Q7; B7ZLR5
• Molecular Weight: 110-116 kDa
• NCBI Official Full Name: slit homolog 2 protein isoform 1
• NCBI Official Synonym Full Names: slit homolog 2 (Drosophila)
• NCBI Official Symbol: SLIT2
• NCBI Official Synonym Symbols: SLIL3; Slit-2
• NCBI Protein Information: slit homolog 2 protein
• UniProt Protein Name: Slit homolog 2 protein
• UniProt Gene Name: SLIT2
• UniProt Synonym Gene Names: SLIL3; Slit-2
• UniProt Entry Name: SLIT2_HUMAN

NCBI Description

This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. Ref.3 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11

Subunit structure: Interacts with GREM1

By similarity. Homodimer. Binds ROBO1 and ROBO2 with high affinity. Ref.3 Ref.10 Ref.15

Subcellular location: Secreted. Note: The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. Ref.3

Tissue specificity: Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined. Ref.1 Ref.2

Domain: The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro. Ref.9

Sequence similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 7 EGF-like domains.Contains 1 laminin G-like domain.Contains 20 LRR (leucine-rich) repeats.Contains 4 LRRCT domains.Contains 4 LRRNT domains.