Rat SLIT2 ELISA Kit | SLIT2 elisa kit
Rat SLIT2 (Slit Homolog 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SLIT2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat SLIT2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SLIT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SLIT2. You can calculate the concentration of Rat SLIT2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Extracellular matrix
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: extracellular space; cell surface; membrane; cytoplasm; plasma membrane; extracellular region
Molecular Function: heparin binding; laminin-1 binding; identical protein binding; protein binding; proteoglycan binding; protein homodimerization activity; Roundabout binding; chemorepellent activity; calcium ion binding; GTPase inhibitor activity
Biological Process: negative regulation of small GTPase mediated signal transduction; axon guidance; positive regulation of apoptosis; negative chemotaxis; motor axon guidance; negative regulation of smooth muscle cell migration; negative regulation of axon extension; negative regulation of cell proliferation; corticospinal neuron axon guidance through the spinal cord; induction of negative chemotaxis; cell-cell adhesion; response to cortisol stimulus; negative regulation of protein amino acid phosphorylation; ureteric bud development; negative regulation of leukocyte chemotaxis; negative regulation of cell migration; in utero embryonic development; negative regulation of actin filament polymerization; chemorepulsion involved in postnatal olfactory bulb interneuron migration; dorsal/ventral axon guidance; cellular response to hormone stimulus; axon extension involved in axon guidance; chemorepulsion involved in embryonic olfactory bulb interneuron migration; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; negative regulation of catalytic activity; negative regulation of vascular permeability; positive regulation of axonogenesis; negative regulation of cell growth; metanephros development; retinal ganglion cell axon guidance
