Enolase Enzyme | eno enzyme
Enolase (Yeast)
Pyruvate kinase: 0.000%
One mL of solution contains the following
K-PO4: 0.05mol/L
Glycerol: 50%
pH: 7.2
NCBI and Uniprot Product Information
NCBI Description
Binds TrxA (Kumar, 2004). [More information is available at EcoGene: EG10258]. Enolase catalyzes the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. [More information is available at EcoCyc: EG10258].
Uniprot Description
Function: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. It is also a component of the RNA degradosome, a multi-enzyme complex involved in RNA processing and messenger RNA degradation. Its interaction with RNase E is important for the turnover of mRNA, in particular on transcripts encoding enzymes of energy-generating metabolic routes. Its presence in the degradosome is required for the response to excess phosphosugar. May play a regulatory role in the degradation of specific RNAs, such as ptsG mRNA, therefore linking cellular metabolic status with post-translational gene regulation. Ref.5 Ref.10 Ref.11
Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O. HAMAP MF_00318
Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.
Enzyme regulation: The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. HAMAP MF_00318
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. HAMAP MF_00318
Subunit structure: Homodimer. Interacts with the C-terminal region of the endoribonuclease RNase E in the RNA degradosome. Ref.14
Subcellular location: Cytoplasm › cytoskeleton. Secreted. Cell surface. Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm, is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. Ref.12
Post-translational modification: Phosphorylated on serine residue(s). Ref.8
Sequence similarities: Belongs to the enolase family.
Research Articles on eno
Similar Products
Product Notes
The eno eno (Catalog #AAA682037) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Enolase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
