Testing Data
Dapi-Fluoromount-G Isotype Control
Dapi-Fluoromount-G
Applications
ELISA, Immunocytochemistry, Immunohistochemistry
Synonyms
Dapi-Fluoromount-G; Dapi-Fluoromount-G isotype control
Form/Format
Clear liquid mounting medium
Applicable Applications for Dapi-Fluoromount-G isotype control
IHC-FS1-9
IHC-PS7,10-14
ICC15-44
(F)ISH3,36,43,45-47
TUNEL7,11,35,48
PLA49,50
Migration21,23,31,33
Apop38
IHC-PS7,10-14
ICC15-44
(F)ISH3,36,43,45-47
TUNEL7,11,35,48
PLA49,50
Migration21,23,31,33
Apop38
Application Notes
Contains sodium azide
Instructions for Use
1. Prepare cytocentrifuge preps according to established procedures.
2. Fix and re-hydrate preps.
3. Remove one slide at a time from aqueous buffer, add 1 drop of Dapi-Fluoromount-G directly to cell prep, mount cover slip and press gently with a gauze sponge to remove excess mounting medium and to seal the cover slip.
4. Allow mounted preps to air dry for 5 minutes before examination.
2. Fix and re-hydrate preps.
3. Remove one slide at a time from aqueous buffer, add 1 drop of Dapi-Fluoromount-G directly to cell prep, mount cover slip and press gently with a gauze sponge to remove excess mounting medium and to seal the cover slip.
4. Allow mounted preps to air dry for 5 minutes before examination.
Suggested Protocol
1:Prepare samples and perform staining according to established procedures
2:Following aqueous final step, remove excess liquid around the sample and add 1 drop (~50 uL) of DAPI Fluoromount-G® for an area of 22 x 22 mm
3: Mount coverslip and remove excess mounting medium
4: Allow to air dry for 5 minutes prior to analysis
2:Following aqueous final step, remove excess liquid around the sample and add 1 drop (~50 uL) of DAPI Fluoromount-G® for an area of 22 x 22 mm
3: Mount coverslip and remove excess mounting medium
4: Allow to air dry for 5 minutes prior to analysis
Preparation and Storage
Dapi-Fluoromount-G is supplied as a 20 mL solution containing 0.1% NaN3 as preservative; Avoid exposure to light and store at ambient tempeture.
Testing Data
Testing Data
Related Product Information for Dapi-Fluoromount-G isotype control
DAPI Fluoromount-G® is a water-soluble, 4',6-diamidino-2-phenylindole (DAPI) containing compound recommended for slides mounted after a staining procedure having an aqueous final step. When bound to DNA, DAPI fluoresces and is typically used as a nuclear/chromosomal counterstain. Since DAPI Fluoromount-G® is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened. This mounting medium also provides a semi-permanent seal for storage of slide preparations.
Similar Products
Product Notes
The Dapi-Fluoromount-G (Catalog #AAA679125) is an Isotype Control and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dapi-Fluoromount-G can be used in a range of immunoassay formats including, but not limited to, IHC-FS1-9 IHC-PS7,10-14 ICC15-44 (F)ISH3,36,43,45-47 TUNEL7,11,35,48 PLA49,50 Migration21,23,31,33 Apop38 . Contains sodium azide. Researchers should empirically determine the suitability of the Dapi-Fluoromount-G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dapi-Fluoromount-G, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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