Mouse IgG1 Isotype Control
Mouse IgG1-UNLB
Applications
ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Synonyms
Mouse IgG1 Isotype Control; Monoclonal Isotype Control; Mouse IgG1-UNLB; Mouse IgG1 - Purified; Unlabeled Antibody; Mouse IgG1 Isotype Control isotype control
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
15H6
Specificity
T-2 mycotoxin
Form/Format
Purified (UNLB) Antibody
Applicable Applications for Mouse IgG1 Isotype Control isotype control
ELISA; FLISA; Flow Cytometry; Immunocytochemistry; Immunohistochemistry.
Application Notes
WORKING DILUTIONS:
Flow Cytometry
Purified (UNLB) antibody and BIOT conjugate: < or = 1ug/10^6 cells
FITC, PE, APC, PE/TXRD, and SPRD conjugates: 10uL/10^6 cells
CY5, PE/CY5.5, PE/CY7, and APC/CY5.5 conjugates: 10uL/10^6 cells
APC/CY7, AF488, AF647, and PACBLU conjugates: 10uL/10^6 cells
ELISA
Purified (UNLB) antibody: 1-5ug/mL
AP and HRP conjugates: 1:2,000-1:4,000
Other Applications:Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.
Flow Cytometry
Purified (UNLB) antibody and BIOT conjugate: < or = 1ug/10^6 cells
FITC, PE, APC, PE/TXRD, and SPRD conjugates: 10uL/10^6 cells
CY5, PE/CY5.5, PE/CY7, and APC/CY5.5 conjugates: 10uL/10^6 cells
APC/CY7, AF488, AF647, and PACBLU conjugates: 10uL/10^6 cells
ELISA
Purified (UNLB) antibody: 1-5ug/mL
AP and HRP conjugates: 1:2,000-1:4,000
Other Applications:Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.
Characterization
To insure lot-to-lot consistency, each batch of product is tested ny ELISA, FLISA, and/or flow cytometry to conform to characteristics fo a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647) conjugates are supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647) conjugates are supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Product Categories/Family for Mouse IgG1 Isotype Control isotype control
Similar Products
Product Notes
The Mouse IgG1 Isotype Control (Catalog #AAA679010) is an Isotype Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mouse IgG1 Isotype Control can be used in a range of immunoassay formats including, but not limited to, ELISA; FLISA; Flow Cytometry; Immunocytochemistry; Immunohistochemistry. WORKING DILUTIONS: Flow Cytometry Purified (UNLB) antibody and BIOT conjugate: < or = 1ug/10^6 cells FITC, PE, APC, PE/TXRD, and SPRD conjugates: 10uL/10^6 cells CY5, PE/CY5.5, PE/CY7, and APC/CY5.5 conjugates: 10uL/10^6 cells APC/CY7, AF488, AF647, and PACBLU conjugates: 10uL/10^6 cells ELISA Purified (UNLB) antibody: 1-5ug/mL AP and HRP conjugates: 1:2,000-1:4,000 Other Applications:Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs. Researchers should empirically determine the suitability of the Mouse IgG1 Isotype Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse IgG1 Isotype Control, Monoclonal Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
