Mouse Anti-Rabbit Light Chain Secondary Antibody
Mouse Anti-Rabbit Light Chain-AP
Reactivity
Rabbit
Applications
ELISA, Western Blot
Synonyms
Mouse Anti-Rabbit Light Chain; Monoclonal Secondary Antibody; Mouse Anti-Rabbit Light Chain-AP; Mouse Anti-Rabbit Light Chain secondary antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG2b lambda
Clone Number
SB62a
Specificity
Rabbit Light Chain
Mouse Anti-Rabbit Light Chain antibody (clone: SB62a) reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50kD). By using our Mouse Anti-Rabbit Light Chain antibody, detection of antigens with molecular weights near 50kD is not obscured by large amounts of reduced and denatured Rabbit IgG heavy chains on the blots. If the antigen of interest has a reduced and denatured molecular weight near 25kD, Mouse Anti-Rabbit IgG Clone: 2A9) may be used to detect the heavy chain of the Rabbit IgG primary antibody without binding to the 25kD band of reduced and denatured IgG Light Chains on Western Blots.
This antibody is not recommended for sensitive or quantitative detection of the reduced and denatured Rabbit Light Chains on Western Blots.
Mouse Anti-Rabbit Light Chain antibody (clone: SB62a) reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50kD). By using our Mouse Anti-Rabbit Light Chain antibody, detection of antigens with molecular weights near 50kD is not obscured by large amounts of reduced and denatured Rabbit IgG heavy chains on the blots. If the antigen of interest has a reduced and denatured molecular weight near 25kD, Mouse Anti-Rabbit IgG Clone: 2A9) may be used to detect the heavy chain of the Rabbit IgG primary antibody without binding to the 25kD band of reduced and denatured IgG Light Chains on Western Blots.
This antibody is not recommended for sensitive or quantitative detection of the reduced and denatured Rabbit Light Chains on Western Blots.
Form/Format
Alkaline Phosphatase (AP) Conjugate
Applicable Applications for Mouse Anti-Rabbit Light Chain secondary antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2,000-1:4,000
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing
Preparation and Storage
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
Similar Products
Product Notes
The Mouse Anti-Rabbit Light Chain (Catalog #AAA675060) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Rabbit Light Chain-AP reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse Anti-Rabbit Light Chain can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2,000-1:4,000. Researchers should empirically determine the suitability of the Mouse Anti-Rabbit Light Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Rabbit Light Chain, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
