Mouse anti-Human CD15 Monoclonal Antibody
Mouse Anti-Human CD15-FITC
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD15; Monoclonal Antibody; Mouse Anti-Human CD15-FITC; Mouse Anti-Human CD15 - FITC; Mouse Anti-Human CD15; anti-CD15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgM
Clone Number
28
Specificity
Human CD15
CD15 is a carbohydrate antigen (also known as Lewis X antigen) found on the cell surface of neutrophils, eosinophils and monocytes, but not on basophils, platelets, lymphocytes or erythrocytes. CD15 may function in adhesion as its ligand is L-selectin (CD62L).1-5
CD15 is a carbohydrate antigen (also known as Lewis X antigen) found on the cell surface of neutrophils, eosinophils and monocytes, but not on basophils, platelets, lymphocytes or erythrocytes. CD15 may function in adhesion as its ligand is L-selectin (CD62L).1-5
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for anti-CD15 antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): Inhibition of neutrophil phagocytosis and bactericial activity
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753.
4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275.
5. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD15 Section, Academic Press, New York, p. 171.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753.
4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275.
5. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD15 Section, Academic Press, New York, p. 171.
