Bu-1a Secondary Antibody

Mouse Anti-Chicken Bu-1a-BIOT

Cat. Num. AAA673946

• Reactivity: Chicken
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: Bu-1a; Monoclonal Secondary Antibody; Mouse Anti-Chicken Bu-1a-BIOT; Mouse Anti-Chicken Bu-1a - Biotin; Mouse Anti-Chicken Bu-1a; Bu-1a secondary antibody

• Host: Mouse
• Reactivity: Chicken
• Clonality: Monoclonal
• Isotype: Mouse (BALB/c) IgG1 kappa
• Clone Number: 21-1A4
• Specificity: Chicken Bu-1a alloantigen, Mr 70 kDa; Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence.1-2
• Form/Format: Biotin (BIOT) Conjugate

• Applicable Applications for Bu-1a secondary antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Immunogen: Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens1
• Characterization: To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are shown on page 2 of this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Veroma, T., O. Vainio, E. Eerola, and P. Toivanen. 1988. Hybridoma 7:41.
2. Houssaint, E., E. Diez, and J.R. Pink. 1987. Immunol. 62:463.
3. Vainio, O. 1998. Personal communication.