Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Rabbit anti-Human, Rat Beclin 1 Polyclonal Antibody | anti-BECN1 antibody
Rabbit Anti-Human Beclin 1 Polyclonal
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601). Lane 1: MW Ladder. Lane 2: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601). Lane 1: MW Ladder. Lane 2: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Protein type: Membrane protein, peripheral; Adaptor/scaffold; Autophagy
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: autophagic vacuole; cytoplasmic membrane-bound vesicle; cytosol; dendrite; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; endosome membrane; extrinsic to membrane; mitochondrial membrane; mitochondrion; nucleus; phagocytic vesicle; trans-Golgi network
Molecular Function: phosphoinositide 3-kinase binding; protein binding; ubiquitin protein ligase binding
Biological Process: apoptosis; autophagic vacuole formation; autophagy; beta-amyloid metabolic process; cellular defense response; cellular response to glucose starvation; cellular response to nitrogen starvation; CVT pathway; cytokinesis; defense response to virus; engulfment of apoptotic cell; late endosome to vacuole transport; lysosome organization and biogenesis; macroautophagy; mitotic metaphase plate congression; negative regulation of apoptosis; negative regulation of cell proliferation; neuron development; positive regulation of autophagy; positive regulation of phosphoinositide 3-kinase cascade; receptor catabolic process; regulation of catalytic activity; regulation of cytokinesis; response to drug; response to hypoxia; response to vitamin E; viral reproduction
Research Articles on BECN1
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Product Notes
The BECN1 becn1 (Catalog #AAA807620) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human Beclin 1 Polyclonal reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Beclin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000. Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beclin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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