Mouse anti-Chicken CD4 Monoclonal Antibody | anti-CD4 antibody

Mouse Anti-Chicken CD4-PE

Cat. Num. AAA673869

• Gene Names: CD4; CD4mut
• Reactivity: Chicken
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD4; Monoclonal Antibody; Mouse Anti-Chicken CD4-PE; Mouse Anti-Chicken CD4 - Phycoerythrin-Labeled; Mouse Anti-Chicken CD4; anti-CD4 antibody

• Host: Mouse
• Reactivity: Chicken
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: CT-4
• Specificity: Chicken CD4 (Mr 64 kDa); CD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 10% of spleen cells and 45% of peripheral blood lymphocytes, but <1% of bursal cells.1 The monoclonal antibody CT-4 inhibits both PHA- and Con A-induced proliferative responses of splenocytes, and PWM-induced IL-2 production.1 This antibody also reacts with turkey CD4 as demonstrated by flow cytometry and immunoprecipitation/western blotting.3
• Form/Format: R - phycoerythrin (R - PE) Conjugate
• Sequence Length: 71

• Applicable Applications for anti-CD4 antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): Inhibition of mitogen-induced proliferation; ; Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.

NCBI and Uniprot Product Information

• NCBI GI #: 3947700
• NCBI GeneID: 920
• UniProt Accession #: Q13969; P01730; Q6LCP8
• Molecular Weight: 7,844 Da
• NCBI Official Full Name: CD4
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); OTTHUMP00000238897; T-cell surface antigen T4/Leu-3
• UniProt Protein Name: CD4 protein
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Synonym Gene Names: CD4 receptor
• UniProt Entry Name: Q13969_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; enzyme binding; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; signal transduction; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation

Disease: Okt4 Epitope Deficiency