Rat anti-Mouse LPAM-1 (Integrin alpha 4 beta 7) Monoclonal Antibody
Rat Anti-Mouse LPAM-1-UNLB
The lymphocyte Peyer's patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7, is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM-1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.1-4 The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer.1 2
Induction of homotypic cellular aggregation
Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
2. Andrew, D. P., L.S. Rott, P.J. Kilshaw, et al. 1996. Eur J. Immunol. 26:897.
3. Hamann, A., et al. 1994. J. Immunol. 152:3282.
4. Berlin, C., E.L. Berg, M.J. Briskin, et al. 1993. Cell 74:185.