Ly49I Secondary Antibody | Klra9 secondary antibody

Mouse Anti-Mouse Ly-49I-PE

Cat. Num. AAA673549

• Gene Names: Klra9; Ly49I; LY49I1
• Reactivity: Mouse
• Applications: Flow Cytometry
• Synonyms: Ly49I; Monoclonal Secondary Antibody; Mouse Anti-Mouse Ly-49I-PE; Mouse Anti-Mouse Ly-49I - Phycoerythrin-Labeled; Mouse Anti-Mouse Ly49I; Klra9 secondary antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse (BALB/c) IgG1 kappa
• Clone Number: YLI-90
• Specificity: B6 allele of the Ly49I receptor; The monoclonal antibody YLI-90 reacts with the B6 allele of Ly49I, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The YLI-90 antibody stains ~ 45% of NK1.1+CD3- splenocytes from C57BL/6 mice.1
• Form/Format: R - phycoerythrin (R - PE) Conjugate
• Sequence Length: 69

• Applicable Applications for Klra9 secondary antibody: Flow Cytometry
• Application Notes: Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
• Characterization: To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Coles, M.C., C.W. McMahon, H. Takizawa, and D.H. Raulet. 2000. Eur. J. Immunol. 30:236.
2. Ljunggren, H.G., and K. Karre. 1990. Immunol. Today 11:237.
3. Liu, S., and D.H. Raulet. Personal communication.

NCBI and Uniprot Product Information

• NCBI GI #: 48476176
• NCBI GeneID: 16640
• UniProt Accession #: Q6IVG0; Q2TJJ8; Q8R4N7; Q9JHN9
• Molecular Weight: 8,034 Da
• NCBI Official Full Name: Ly49I receptor
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily A, member 9
• NCBI Official Symbol: Klra9
• NCBI Official Synonym Symbols: Ly49I; LY49I1
• NCBI Protein Information: killer cell lectin-like receptor subfamily A, member 9; Ly49I receptor; OTTMUSP00000026553; OTTMUSP00000026554
• UniProt Protein Name: Ly49I receptor
• UniProt Gene Name: Klra9
• UniProt Synonym Gene Names: Klra7
• UniProt Entry Name: Q6IVG0_MOUSE

Uniprot Description

CLEC1B: Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. Acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction; platelet formation; signal transduction