Rat anti-Mouse CD11b/Mac-1 Monoclonal Antibody | anti-ITGAM antibody
Rat Anti-Mouse CD11b/Mac-1a-FITC
Gene Names
ITGAM; CR3; C3bi; CD11B
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD11b/Mac-1; Monoclonal Antibody; Rat Anti-Mouse CD11b/Mac-1a-FITC; Rat Anti-Mouse CD11b/Mac-1a - FITC; Rat Anti-Mouse CD11b/Mac-1; anti-ITGAM antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
3A33
Specificity
alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer>br>CD11b (integrin alpha M subunit) combines with CD18 (integrin beta 2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54).^2-6 Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities.^2, 7-10 In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process,^4, 11 Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.^12
Form/Format
Fluorescein (FITC) Conjugate
Sequence Length
1152
Applicable Applications for anti-ITGAM antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and Allophycocyanin-Cyanine 7 (APCCY 7)conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and Allophycocyanin-Cyanine 7 (APCCY 7)conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Martin, A., R. Le Corre, P. Pellen,, D. Bourel, G. Merdrignac, B. Genetet, and L. Toujas. 1986. Tissue Antigens 28:15.
2. Springer, T., G. Galfre, D.S. Secher, and C. Milstein. 1979. Eur. J. Immunol. 9:301.
3. Springer, T.A., D. Davignon, M.-K. Ho, K. Kurzinger, E. Martz, and F. Sanchez-Madrid. 1982. Immunol. Rev. 68:171.
4. Beller, D.I., T.A. Springer, R.D. Schreiber. 1982. J. Exp. Med. 156:1000.
5. Sanchez-Madrid, F., P. Simon, S. Thompson, and T.A. Springer. 1983. J. Exp. Med. 158:586.
6. Lub, M., Y. van Kooyk, and C.J. Figdor. 1996. J. Leukoc. Biol. 59:648.
7. Kantor, A.B., A. M. Stall, S. Adams, L.A. Herzenberg, and L.A. Herzenberg. 1992. Proc. Natl. Acad. Sci. USA 89:3320.
8. Ault, K.A., and T.A. Springer. 1981. J. Immunol. 126:359.
9. Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shrotman. 1992. J. Exp. Med. 176:47.
10. Leenen, P.J. M., M.F.T.R. de Bruijn, J.S.A. Voerman, P.A. Campbell, and W. van Ewijk. 1994. J. Immunol. Meth. 174:5.
11. Gresham, H.D., et al. 1991. J. Clin. Invest. 88:588.
12. Springer, T.A. 1994. Cell 76:310.
2. Springer, T., G. Galfre, D.S. Secher, and C. Milstein. 1979. Eur. J. Immunol. 9:301.
3. Springer, T.A., D. Davignon, M.-K. Ho, K. Kurzinger, E. Martz, and F. Sanchez-Madrid. 1982. Immunol. Rev. 68:171.
4. Beller, D.I., T.A. Springer, R.D. Schreiber. 1982. J. Exp. Med. 156:1000.
5. Sanchez-Madrid, F., P. Simon, S. Thompson, and T.A. Springer. 1983. J. Exp. Med. 158:586.
6. Lub, M., Y. van Kooyk, and C.J. Figdor. 1996. J. Leukoc. Biol. 59:648.
7. Kantor, A.B., A. M. Stall, S. Adams, L.A. Herzenberg, and L.A. Herzenberg. 1992. Proc. Natl. Acad. Sci. USA 89:3320.
8. Ault, K.A., and T.A. Springer. 1981. J. Immunol. 126:359.
9. Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shrotman. 1992. J. Exp. Med. 176:47.
10. Leenen, P.J. M., M.F.T.R. de Bruijn, J.S.A. Voerman, P.A. Campbell, and W. van Ewijk. 1994. J. Immunol. Meth. 174:5.
11. Gresham, H.D., et al. 1991. J. Clin. Invest. 88:588.
12. Springer, T.A. 1994. Cell 76:310.
NCBI and Uniprot Product Information
Research Articles on ITGAM
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Product Notes
The ITGAM (Catalog #AAA673208) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD11b/Mac-1a-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b/Mac-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the ITGAM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b/Mac-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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